Ziege anti-Maus IgG1 Antikörper (HRP)

Produktnummer ABIN1118471

Hersteller Produkt- Nr.: 610-103-040

Kurzübersicht für Ziege anti-Maus IgG1 Antikörper (HRP) (ABIN1118471)

Target: IgG1

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Isotyp: IgG

Produktdetails

Reaktivität: Maus

Wirt: Ziege

Hersteller Produkt- Nr.: 610-103-040

Hersteller: Rockland

Verwendungszweck: Mouse IgG1 Antibody Peroxidase Conjugated Pre-adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Bv, Hu, and Rb Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse IgG1. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1 % of target signal.

Produktmerkmale: Anti-Mouse IgG IgA IgM Fluorescein Antibody generated in goat detects reactivity to Mouse IgG, Mouse IgA, and Mouse IgM.

Immunogen:

Immunogen: Mouse IgG1 heavy chain

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Mouse IgG1 Peroxidase Antibody has been tested by ELISA and is ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:20,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: IgG1

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.