Ziege anti-Armenischer Hamster IgG (Heavy & Light Chain) Antikörper (HRP) - Preadsorbed

Produktnummer ABIN1044078

Hersteller Produkt- Nr.: 720-103-440

Produktdetails

Target: IgG

Fragment: F(ab')2 fragment

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Syrischer Goldhamster, Armenischer Hamster

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Hersteller Produkt- Nr.: 720-103-440

Hersteller: Rockland

Verwendungszweck: F(Ab')2 Fragment Of Hamster IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against MOUSE and RAT Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Golden Syrian and Armenian Hamster IgG and Golden Syrian and Armenian Hamster Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) and Mouse or Rat Serum Proteins.

Produktmerkmale: F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG Fluorescein Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.

Aufreinigung: Preadsorption: Pre-Adsorbed

Immunogen: Optional[Immunogen]: Golden Syrian and Armenian Hamster IgG whole molecules

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Application Note: F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG Peroxidase Antibody has been tested by ELISA and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund: Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.