Esel anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (TRITC) - Preadsorbed

Produktnummer ABIN1044054

Hersteller Produkt- Nr.: 711-700-127

Kurzübersicht für Esel anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (TRITC) - Preadsorbed (ABIN1044054)

Target: IgG

Fragment: F(ab')2 fragment

Klonalität: Polyklonal

Konjugat: TRITC

Applikation: Western Blotting (WB), Fluorescence Microscopy (FM)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Kaninchen

Wirt: Esel

Hersteller Produkt- Nr.: 711-700-127

Hersteller: Rockland

Verwendungszweck: F(ab')2 Rabbit IgG Antibody Rhodamine Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

Produktmerkmale: F(ab')2 Anti-Rabbit IgG (H&L) Antibody generated in goat detects rabbit IgG. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This F(ab')2 Anti-Rabbit IgG Antibody is conjugated to Texas Red™.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Immunogen: Optional[Immunogen]: Rabbit IgG whole molecule

Anwendungsinformationen

Applikationshinweise: Application Note: F(ab')2 Anti-Rabbit IgG Rhodamine Conjugated Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. IF Microscopy Dilution: 1:500-1:2,500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

Konzentration: 1.0 mg/mL

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: F(ab')2 Anti-Rabbit IgG (H&L) Antibody generated in donkey detects rabbit IgG. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This F(ab')2 Anti-Rabbit IgG Antibody is conjugated to Rhodamine.