Kaninchen anti-Cat IgG (Heavy & Light Chain) Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN1044007

Hersteller Produkt- Nr.: 702-405-002

Produktdetails

Target: IgG

Fragment: F(ab')2 fragment

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Cat

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Hersteller Produkt- Nr.: 702-405-002

Hersteller: Rockland

Verwendungszweck: F(ab')2 Cat IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Cat IgG and Cat Serum.  No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).

Produktmerkmale: F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Sterilität: Sterile filtered

Immunogen: Optional[Immunogen]: Cat IgG whole molecule

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Application Note: F(ab')2 Anti-Cat IgG Alkaline Phosphatase Conjugated Antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Cat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:200-1:1,000 Western Blot Dilution: 1:500-1:2,500 ELISA Dilution: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Optional[Buffer]: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund: Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.