Ziege anti-Armenischer Hamster IgG (Heavy & Light Chain) Antikörper (Biotin)
Kurzübersicht für Ziege anti-Armenischer Hamster IgG (Heavy & Light Chain) Antikörper (Biotin) (ABIN1043999)
Target
Klonalität
Konjugat
Applikation
Isotyp
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Bindungsspezifität
- Heavy & Light Chain
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Reaktivität
- Syrischer Goldhamster, Armenischer Hamster
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Wirt
- Ziege
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Hersteller Produkt- Nr.
- 620-106-440
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Hersteller
- Rockland
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Verwendungszweck
- Golden Syrian & Armenian Hamster IgG Antibody Biotin Conjugated
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Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Armenian/Golden Syrian Hamster IgG and Armenian/Golden Syrian Hamster Serum. No reaction was observed against Mouse or Rat Serum Proteins.
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Produktmerkmale
- Anti-Golden Syrian & Armenian Hamster IgG Alkaline Phosphatase Antibody generated in goat detects Golden Syrian & Armenian Hamster IgG.
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Aufreinigung
- Anti-Golden Syrian and Armenian hamster IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian/Golden Syrian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Immunogen
- Optional[Immunogen]: Armenian and Golden Syrian Hamster IgG, whole molecule
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Applikationshinweise
- Application Note: Anti Golden Syrian & Armenian Hamster IgG Biotin conjugated antibody has been tested by ELISA and western blot and is suitable for Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Immunohistochemistry Dilution: 1:500 - 1:2,000 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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Konzentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Haltbarkeit
- 12 months
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- IgG
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Substanzklasse
- Antibody
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Hintergrund
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Target
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