Ziege anti-Maus IgG3 Antikörper

Produktnummer ABIN1043980

Hersteller Produkt- Nr.: 610-101-043

Produktdetails

Target: IgG3

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 610-101-043

Hersteller: Rockland

Verwendungszweck: Mouse IgG3 Antibody Pre-adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against to Bv, Hu & Rb Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum, Mouse IgG and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA for human and rabbit. For IgG1, IgG2a and IgG2b reactivity was less than 5 % against target (IgG3, IgG). Cross-reactivity against Bovine is assessed only by IEP.

Produktmerkmale: Anti-Mouse IgG2b Antibody generated in goat detects reactivity to Mouse IgG2b (Gamma 2b chain).

Aufreinigung: Anti-MOUSE IgG3 (Gamma 3 chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG3 coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Mouse IgG3 heavy chain

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Mouse IgG3 secondary antibody has been tested by ELISA and is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG3 antibody based assays. Mouse IgG3 secondary antibody is available in a variety of formats. Immunohistochemistry Dilution: 1:2,000 - 1:10,000 Western Blot Dilution: 1:4,000 - 1:20,000 ELISA Dilution: 1:40,000 - 1:200,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Antigendetails

Target: IgG3

Abstract: IgG3 Produkte

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG3 comprises almost 10 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.