Ziege anti-Human IgG (Heavy & Light Chain) Antikörper (HRP) - Preadsorbed
Kurzübersicht für Ziege anti-Human IgG (Heavy & Light Chain) Antikörper (HRP) - Preadsorbed (ABIN1043965)
Target
Klonalität
Konjugat
Applikation
Isotyp
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Bindungsspezifität
- Heavy & Light Chain
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Reaktivität
- Human
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Wirt
- Ziege
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Hersteller Produkt- Nr.
- 609-1316-0100
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Hersteller
- Rockland
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Verwendungszweck
- Human IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed
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Kreuzreaktivität (Details)
- Minimal crossreactivity against MOUSE Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against Mouse Serum Proteins.
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Produktmerkmale
- Anti-Human IgG (gamma chain) Peroxidase generated in goat detects human Immunoglobulin G (gamma chain).
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Aufreinigung
- Human IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Immunogen
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Immunogen: Human IgG whole molecule
Immunogen Type: Native Protein
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Applikationshinweise
- Application Note: Anti-Human IgG (H&L) Peroxidase Conjugate has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:2,000 - 1:20,000 ELISA Dilution: 1:400,000 - 1:600,000 Other: User Optimized
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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Konzentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
Konservierungsmittel
- Gentamicin sulfate
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Vorsichtsmaßnahmen
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Haltbarkeit
- 12 months
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Comparison of Enzyme-Linked Immunosorbent Assay Using Either Natural Octyl Disaccharide-Leprosy IDRI Diagnostic or Phenolic Glycolipid-I Antigens for the Detection of Leprosy Patients in Colombia." in: The American journal of tropical medicine and hygiene, Vol. 98, Issue 1, pp. 274-277, (2018) (PubMed).
: "
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Comparison of Enzyme-Linked Immunosorbent Assay Using Either Natural Octyl Disaccharide-Leprosy IDRI Diagnostic or Phenolic Glycolipid-I Antigens for the Detection of Leprosy Patients in Colombia." in: The American journal of tropical medicine and hygiene, Vol. 98, Issue 1, pp. 274-277, (2018) (PubMed).
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- IgG
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Substanzklasse
- Antibody
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Hintergrund
- It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Target
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