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Esel anti-Meerschweinchen IgG (Heavy & Light Chain) Antikörper - Preadsorbed

ELISA, WB Polyclonal IgG unconjugated
Rockland
Produktnummer ABIN1043951
Hersteller Produkt- Nr.: 606-701-c36

Kurzübersicht für Esel anti-Meerschweinchen IgG (Heavy & Light Chain) Antikörper - Preadsorbed (ABIN1043951)

Target

IgG

Klonalität

  • 3453
  • 231
  • 16
  • 1
Polyklonal

Applikation

  • 2119
  • 2053
  • 1900
  • 1873
  • 999
  • 920
  • 896
  • 520
  • 424
  • 234
  • 234
  • 149
  • 102
  • 98
  • 90
  • 84
  • 51
  • 33
  • 30
  • 28
  • 25
  • 20
  • 15
  • 11
  • 9
  • 8
  • 7
  • 7
  • 5
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Western Blotting (WB)

Isotyp

IgG
  • Bindungsspezifität

    • 2396
    • 517
    • 417
    • 146
    • 130
    • 128
    • 66
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Heavy & Light Chain

    Reaktivität

    • 752
    • 706
    • 609
    • 474
    • 410
    • 243
    • 213
    • 176
    • 146
    • 141
    • 124
    • 110
    • 107
    • 101
    • 83
    • 62
    • 48
    • 33
    • 28
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Meerschweinchen

    Wirt

    • 1787
    • 1583
    • 568
    • 284
    • 122
    • 112
    • 41
    • 32
    • 22
    • 21
    • 15
    • 11
    • 11
    Esel

    Hersteller Produkt- Nr.

    606-701-c36

    Hersteller

    Rockland

    Verwendungszweck

    Guinea Pig IgG (H&L) Antibody Pre-Adsorbed

    Kreuzreaktivität (Details)

    Minimal crossreactivity against Bv Ch Gt Ham Hs Hu Ms Rb Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Guinea Pig IgG and Guinea Pig Serum.   No reaction was observed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Guinea Pig IgG and with light chains of most Guinea Pig immunoglobulins.

    Produktmerkmale

    Anti-Guinea Pig IgG Rhodamine Antibody generated in donkey detects guinea pig IgG.

    Aufreinigung

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above.

    Sterilität

    Sterile filtered

    Immunogen

    Optional[Immunogen]: Guinea Pig IgG whole molecule
  • Applikationshinweise

    Application Note: Anti-Guinea Pig IgG antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:25,000 - 1:75,000

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    , Preservative:None

    Konservierungsmittel

    Without preservative

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Target

    IgG

    Substanzklasse

    Antibody

    Hintergrund

    Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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