Ziege anti-Kaninchen IgM Antikörper (HRP)

Produktnummer ABIN102696

Hersteller Produkt- Nr.: 211-1307

Kurzübersicht für Ziege anti-Kaninchen IgM Antikörper (HRP) (ABIN102696)

Target: IgM

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Reaktivität: Kaninchen

Wirt: Ziege

Hersteller Produkt- Nr.: 211-1307

Hersteller: Rockland

Verwendungszweck: Rabbit IgM (mu) Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgM and Rabbit Serum. No reaction was observed against Rabbit IgG.

Produktmerkmale: Anti-Rabbit IgM mu chain antibody raised in goat specifically detects rabbit IgM heavy chain.

Aufreinigung: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by cross adsorption to remove any unwanted reactivities and extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-Rabbit IgM (mu) was produced by repeated immunization with rabbit IgM mu heavy chain in goat.

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Rabbit IgM (mu) peroxidase conjugated antibody has been tested by ELISA and is assayed against 1.0 μg of Rabbit IgM in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:20,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL

Konzentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgM

Substanzklasse: Antibody

Hintergrund: Anti-Rabbit IgM (mu) peroxidase conjugated antibody generated in goat detects specifically rabbit IgM (mu). This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.