Ziege anti-Maus IgM Antikörper

Produktnummer ABIN102655

Hersteller Produkt- Nr.: 110-1107

Kurzübersicht für Ziege anti-Maus IgM Antikörper (ABIN102655)

Target: IgM

Klonalität: Polyklonal

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Produktdetails

Reaktivität: Maus

Wirt: Ziege

Hersteller Produkt- Nr.: 110-1107

Hersteller: Rockland

Verwendungszweck: Mouse IgM (H&L) Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in predominantly a single precipitin arc against anti-goat serum, Mouse IgM and Mouse Serum.  No reaction was observed against Mouse IgG F(c).  Some light chain cross reactivity may occur against Mouse IgG.

Produktmerkmale: Anti-Mouse IgG F(ab')2 Antibody generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind mouse IgG F(c) will not bind mouse IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Aufreinigung: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Optional[Immunogen]: Mouse IgM whole molecule

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Mouse IgM antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL

Konzentration: 85 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgM

Substanzklasse: Antibody

Hintergrund: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.