Ziege anti-Huhn IgM Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN102564

Hersteller Produkt- Nr.: 603-105-007

Kurzübersicht für Ziege anti-Huhn IgM Antikörper (Alkaline Phosphatase (AP)) (ABIN102564)

Target: IgM

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Reaktivität: Huhn

Wirt: Ziege

Hersteller Produkt- Nr.: 603-105-007

Hersteller: Rockland

Verwendungszweck: Chicken IgM (mu chain) Antibody Alkaline Phosphatase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Chicken IgM and Chicken Serum.  No reaction was observed against other chicken heavy or light chain proteins.

Produktmerkmale: Anti-Chicken IgG Alkaline Phosphatase Antibody generated in goat detects chicken IgY.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen: Optional[Immunogen]: Chicken IgM whole molecule

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Chicken IgM Alkaline Phosphatase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:200 - 1:2,000 ELISA Dilution: 1:8,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Haltbarkeit: 12 months

Antigendetails

Target: IgM

Substanzklasse: Antibody

Hintergrund: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Chicken IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.