Kaninchen anti-Maus IgG1 Antikörper (Biotin)

Produktnummer ABIN102335

Hersteller Produkt- Nr.: 610-4640

Kurzübersicht für Kaninchen anti-Maus IgG1 Antikörper (Biotin) (ABIN102335)

Target: IgG1

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Isotyp: IgG

Produktdetails

Reaktivität: Maus

Wirt: Kaninchen

Hersteller Produkt- Nr.: 610-4640

Hersteller: Rockland

Verwendungszweck: Mouse IgG1 Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.  Specificity was confirmed by ELISA.  Typically, less than 1 % cross-reactivity was observed against other Mouse heavy chain isotypes.

Produktmerkmale: Anti-Mouse IgG F(ab')2 Biotin antibody generated in rabbit recognizes the dimeric Fab portion of the mouse IgG molecule. Mouse IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind mouse IgG F(c) will not bind mouse IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Aufreinigung: Mouse IgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Optional[Immunogen]: Mouse IgG1 heavy chain

Anwendungsinformationen

Applikationshinweise: Application Note: Mouse IgG1 secondary antibody conjugated to Biotin is available in a variety of formats. Anti-Mouse IgG1 Biotin Antibody has been tested by ELISA and western blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays. Immunohistochemistry Dilution: 1:500 - 1:3,000 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:250,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgG1

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.