Kaninchen anti-Schaf IgG (Fc Region) Antikörper (FITC) - Preadsorbed

Produktnummer ABIN102300

Hersteller Produkt- Nr.: 613-4229

Kurzübersicht für Kaninchen anti-Schaf IgG (Fc Region) Antikörper (FITC) - Preadsorbed (ABIN102300)

Target: IgG

Klonalität: Polyklonal

Konjugat: FITC

Applikation: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Fc Region

Reaktivität: Schaf

Wirt: Kaninchen

Hersteller Produkt- Nr.: 613-4229

Hersteller: Rockland

Verwendungszweck: Sheep IgG Fc Antibody Fluorescein Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum.  No reaction was observed against Sheep IgG F(ab) or Human Serum Proteins.

Produktmerkmale: FITC Conjugated Secondary Antibody is designed for flow cytometry, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Optional[Immunogen]: Sheep IgG F(c) fragment

Anwendungsinformationen

Applikationshinweise: Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Receptors bind the Fc portion of sheep IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.