Kaninchen anti-Schaf IgG (Heavy & Light Chain) Antikörper (HRP) - Preadsorbed
Kurzübersicht für Kaninchen anti-Schaf IgG (Heavy & Light Chain) Antikörper (HRP) - Preadsorbed (ABIN102262)
Target
Klonalität
Konjugat
Applikation
Isotyp
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Bindungsspezifität
- Heavy & Light Chain
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Reaktivität
- Schaf
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Wirt
- Kaninchen
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Hersteller Produkt- Nr.
- 613-4328
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Hersteller
- Rockland
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Verwendungszweck
- Sheep IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed
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Kreuzreaktivität (Details)
- Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Sheep IgG and Sheep Serum. No reaction was observed against Human Serum Proteins.
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Produktmerkmale
- Anti-Sheep IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the sheep IgG molecule. Sheep IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind sheep IgG F(c) will not bind sheep IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Sheep IgG F(ab')2 is conjugated to Peroxidase.
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Aufreinigung
- Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Immunogen
- Optional[Immunogen]: Sheep IgG whole molecule
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Applikationshinweise
- Application Note: Anti-Sheep IgG Peroxidase Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. Immunohistochemistry Dilution: 1:300 - 1:1,500 Western Blot Dilution: 1:500 - 1:3,000 ELISA Dilution: 1:5,000 - 1:22,000 Other: User Optimized
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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Konzentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
Konservierungsmittel
- Gentamicin sulfate
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Vorsichtsmaßnahmen
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Haltbarkeit
- 12 months
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Nuclear expression of thymidylate synthase in colorectal cancer cell lines and clinical samples." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 54, Issue 1, pp. 19-29, (2006) (PubMed).
: "Role for c-jun N-terminal kinase in treatment-refractory acute myeloid leukemia (AML): signaling to multidrug-efflux and hyperproliferation." in: Leukemia, Vol. 16, Issue 5, pp. 799-812, (2002) (PubMed).
: "
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Nuclear expression of thymidylate synthase in colorectal cancer cell lines and clinical samples." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 54, Issue 1, pp. 19-29, (2006) (PubMed).
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- IgG
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Substanzklasse
- Antibody
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Hintergrund
- HRP Conjugated Secondary Antibody is designed for western blotting, ELISA and Immunohistochemistry. Horseradish peroxidase conjugated antibodies are ideal for antibody detection assays that require lot-to-lot consistency.
Target
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