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Kaninchen anti-Schaf IgG (Heavy & Light Chain) Antikörper (HRP) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG HRP
Rockland
Produktnummer ABIN102262
Hersteller Produkt- Nr.: 613-4328

Kurzübersicht für Kaninchen anti-Schaf IgG (Heavy & Light Chain) Antikörper (HRP) - Preadsorbed (ABIN102262)

Target

IgG

Klonalität

  • 3453
  • 231
  • 16
  • 1
Polyklonal

Konjugat

  • 666
  • 423
  • 421
  • 400
  • 274
  • 262
  • 235
  • 119
  • 105
  • 72
  • 67
  • 64
  • 61
  • 58
  • 58
  • 50
  • 44
  • 43
  • 40
  • 40
  • 39
  • 39
  • 36
  • 36
  • 34
  • 32
  • 31
  • 30
  • 29
  • 29
  • 29
  • 29
  • 29
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 27
  • 26
  • 26
  • 25
  • 21
  • 21
  • 19
  • 17
  • 17
  • 16
HRP

Applikation

  • 2119
  • 2052
  • 1900
  • 1873
  • 999
  • 920
  • 896
  • 520
  • 424
  • 234
  • 234
  • 149
  • 102
  • 98
  • 90
  • 84
  • 51
  • 33
  • 30
  • 28
  • 25
  • 20
  • 15
  • 11
  • 9
  • 8
  • 7
  • 7
  • 5
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp

IgG
  • Bindungsspezifität

    • 2396
    • 517
    • 417
    • 146
    • 130
    • 128
    • 66
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Heavy & Light Chain

    Reaktivität

    • 752
    • 706
    • 609
    • 474
    • 410
    • 244
    • 212
    • 176
    • 146
    • 141
    • 124
    • 110
    • 107
    • 101
    • 83
    • 62
    • 48
    • 33
    • 28
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Schaf

    Wirt

    • 1787
    • 1582
    • 569
    • 284
    • 122
    • 112
    • 41
    • 32
    • 22
    • 21
    • 15
    • 11
    • 11
    Kaninchen

    Hersteller Produkt- Nr.

    613-4328

    Hersteller

    Rockland

    Verwendungszweck

    Sheep IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

    Kreuzreaktivität (Details)

    Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Sheep IgG and Sheep Serum.  No reaction was observed against Human Serum Proteins.

    Produktmerkmale

    Anti-Sheep IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the sheep IgG molecule. Sheep IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind sheep IgG F(c) will not bind sheep IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Sheep IgG F(ab')2 is conjugated to Peroxidase.

    Aufreinigung

    Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    Immunogen

    Optional[Immunogen]: Sheep IgG whole molecule
  • Applikationshinweise

    Application Note: Anti-Sheep IgG Peroxidase Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. Immunohistochemistry Dilution: 1:300 - 1:1,500 Western Blot Dilution: 1:500 - 1:3,000 ELISA Dilution: 1:5,000 - 1:22,000 Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

    Konzentration

    1.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

    Konservierungsmittel

    Gentamicin sulfate

    Vorsichtsmaßnahmen

    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store secondary antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Bissoon-Haqqani, Moyana, Jonker, Maroun, Birnboim: "Nuclear expression of thymidylate synthase in colorectal cancer cell lines and clinical samples." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 54, Issue 1, pp. 19-29, (2006) (PubMed).

    Cripe, Gelfanov, Smith, Spigel, Phillips, Gabig, Jung, Fyffe, Hartman, Kneebone, Mercola, Burgess, Boswell: "Role for c-jun N-terminal kinase in treatment-refractory acute myeloid leukemia (AML): signaling to multidrug-efflux and hyperproliferation." in: Leukemia, Vol. 16, Issue 5, pp. 799-812, (2002) (PubMed).

  • Target

    IgG

    Substanzklasse

    Antibody

    Hintergrund

    HRP Conjugated Secondary Antibody is designed for western blotting, ELISA and Immunohistochemistry. Horseradish peroxidase conjugated antibodies are ideal for antibody detection assays that require lot-to-lot consistency.
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