Esel anti-Schaf IgG (Heavy & Light Chain) Antikörper

Produktnummer ABIN102230

Hersteller Produkt- Nr.: 613-701-168

Kurzübersicht für Esel anti-Schaf IgG (Heavy & Light Chain) Antikörper (ABIN102230)

Target: IgG

Klonalität: Polyklonal

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Schaf

Wirt: Esel

Hersteller Produkt- Nr.: 613-701-168

Hersteller: Rockland

Verwendungszweck: Sheep IgG (H&L) Antibody Pre-adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Ch GP Ham Hs Hu Ms Rb & Rt Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Sheep IgG and Sheep Serum.  No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins.

Produktmerkmale: Anti-Sheep IgG (H&L) Rhodamine Antibody generated in donkey detects reactivity to sheep IgG.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Sheep IgG (H&L) was produced by repeated immunization with Sheep IgG whole molecule in donkey.

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Sheep IgG (H&L) has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:39,200 - 1:59,200 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Anti-Sheep IgG antibody generated in donkey detects specifically sheep IgG. This secondary antibody anti-Sheep is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.