Ziege anti-Kaninchen IgG (Fc Region) Antikörper (Alkaline Phosphatase (AP)) - Preadsorbed

Produktnummer ABIN102080

Hersteller Produkt- Nr.: 611-1523

Kurzübersicht für Ziege anti-Kaninchen IgG (Fc Region) Antikörper (Alkaline Phosphatase (AP)) - Preadsorbed (ABIN102080)

Target: IgG

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Fc Region

Reaktivität: Kaninchen

Wirt: Ziege

Hersteller Produkt- Nr.: 611-1523

Hersteller: Rockland

Verwendungszweck: Rabbit IgG Fc Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum.  No reaction was observed against Rabbit IgG F(ab)or Human Serum Proteins.

Produktmerkmale: Anti-Rabbit IgG (H&L) Alkaline Phosphatase Antibody generated in goat detects reactivity to Rabbit IgG.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen: Optional[Immunogen]: Rabbit IgG F(c) fragment

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Rabbit IgG F(c) alkaline phosphatase antibody has been tested by ELISA and dot blot and is assayed against 1.0 μg of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3,000 to 1:15,000 is suggested for this product. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.