Ziege anti-Kaninchen IgG (Fc Region) Antikörper - Preadsorbed
Kurzübersicht für Ziege anti-Kaninchen IgG (Fc Region) Antikörper - Preadsorbed (ABIN102079)
Target
Klonalität
Applikation
Isotyp
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Bindungsspezifität
- Fc Region
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Reaktivität
- Kaninchen
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Wirt
- Ziege
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Hersteller Produkt- Nr.
- 611-1123
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Hersteller
- Rockland
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Verwendungszweck
- Rabbit IgG Fc Antibody Pre-Adsorbed
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Kreuzreaktivität (Details)
- Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab) or Human Serum Proteins.
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Produktmerkmale
- Anti-Rabbit IgG (H&L) Antibody generated in goat detects reactivity to Rabbit IgG.
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Aufreinigung
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Sterilität
- Sterile filtered
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Immunogen
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Immunogen: Anti Rabbit IgG F(c) was produced by repeated immunization with rabbit IgG F(c) fragment in goat.
Immunogen Type: Native Protein
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Applikationshinweise
- Application Note: Anti-Rabbit IgG F(c) is tested by immunoelectrophoresis, ELISA, dot blot, and western blot. This product is suitable for use in western-blot, competitive western-blot, and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. Expect ~25 kDa for Rabbit IgG F(c) Fragment. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 2 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide -
Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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Haltbarkeit
- 12 months
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- IgG
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Substanzklasse
- Antibody
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Hintergrund
- Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
Target
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