Ratte anti-Kaninchen IgG (Heavy & Light Chain) Antikörper - Preadsorbed

Produktnummer ABIN101924

Hersteller Produkt- Nr.: 611-501-122

Kurzübersicht für Ratte anti-Kaninchen IgG (Heavy & Light Chain) Antikörper - Preadsorbed (ABIN101924)

Target: IgG

Klonalität: Polyklonal

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Kaninchen

Wirt: Ratte

Hersteller Produkt- Nr.: 611-501-122

Hersteller: Rockland

Verwendungszweck: Rabbit IgG (H&L) Antibody Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Hu, Gt, Ms Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against Goat, Human and Mouse Serum Proteins.

Produktmerkmale: Anti-Rabbit IgG Antibody Rhodamine generated in rat detects rabbit IgG.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen: Optional[Immunogen]: Rabbit IgG whole molecule

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Rabbit IgG antibody has been tested by ELISA and western blot and is suitable for use in immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:20,000 - 1:100,000 ELISA Dilution: 1:900,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Anti-Rabbit IgG (H&L) generated in rat detects rabbit Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.