Kaninchen anti-Schwein IgG (Heavy & Light Chain) Antikörper
Kurzübersicht für Kaninchen anti-Schwein IgG (Heavy & Light Chain) Antikörper (ABIN101884)
Target
Klonalität
Applikation
Isotyp
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Bindungsspezifität
- Heavy & Light Chain
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Reaktivität
- Schwein
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Wirt
- Kaninchen
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Hersteller Produkt- Nr.
- 614-4102
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Hersteller
- Rockland
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Verwendungszweck
- Swine IgG (H&L) Antibody
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Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Swine IgG, and Swine Serum.
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Produktmerkmale
- Anti-Swine IgG F(ab')2 Rhodamine Antibody generated in rabbit recognizes the dimeric Fab portion of the swine IgG molecule. Swine IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind swine IgG F(c) will not bind swine IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Swine IgG F(ab')2 antibody is conjugated to Rhodamine.
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Aufreinigung
- Anti-SWINE IgG (H&L) (RABBIT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Sterilität
- Sterile filtered
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Immunogen
- Optional[Immunogen]: Swine IgG whole molecule
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Applikationshinweise
- Application Note: Anti-SWINE IgG (H&L) (RABBIT) Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Specific conditions should be optimized by user. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 8.8 mg/mL
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Buffer
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide -
Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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Haltbarkeit
- 12 months
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- IgG
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Substanzklasse
- Antibody
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Hintergrund
- SWINE IgG (H&L) (RABBIT) Antibody generated in rabbit detects specifically swine IgG whole molecule. Anti-Swine IgG antibody is ideal for investigators involved in serum protein component research.
Target
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