Kaninchen anti-Human IgG Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN101667

Hersteller Produkt- Nr.: 609-4503

Kurzübersicht für Kaninchen anti-Human IgG Antikörper (Alkaline Phosphatase (AP)) (ABIN101667)

Target: IgG

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Isotyp: IgG

Produktdetails

Reaktivität: Human

Wirt: Kaninchen

Hersteller Produkt- Nr.: 609-4503

Hersteller: Rockland

Verwendungszweck: Human IgG Fc Antibody Alkaline Phosphatase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum.  No reaction was observed against Human IgG F(ab).

Produktmerkmale: Anti-Human IgG Alkaline Phosphatase Antibody generated in rabbit detects human Immunoglobulin G (IgG).

Aufreinigung: Anti-Human IgG F(c) was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Human IgG F(c) was produced by repeated immunization with Human Fc fragment in rabbit.

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Human IgG F(c) Alkaline Phosphatase Conjugated antibody has been tested by dot blot and ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Receptors bind the Fc portion of human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(c) Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.