Kaninchen anti-Human IgG (F(ab')2 Region) Antikörper

Produktnummer ABIN101627

Hersteller Produkt- Nr.: 609-4118

Kurzübersicht für Kaninchen anti-Human IgG (F(ab')2 Region) Antikörper (ABIN101627)

Target: IgG

Klonalität: Polyklonal

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Isotyp: IgG

Produktdetails

Bindungsspezifität: F(ab')2 Region

Reaktivität: Human

Wirt: Kaninchen

Hersteller Produkt- Nr.: 609-4118

Hersteller: Rockland

Verwendungszweck: Human IgG F(ab')2 Antibody pre-absorbed

Kreuzreaktivität (Details): Minimal crossreactivity against MOUSE Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG, Human IgG F(ab')2 and Human Serum.  No reaction was observed against Human IgG F(c) or Mouse Serum Proteins.

Produktmerkmale: Anti-Human IgG (H&L) generated in rabbit detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present.

Aufreinigung: Anti-Human IgG F(ab')2 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen: Optional[Immunogen]: Anti-Human IgG F(ab')2 fragment was produced in rabbit by repeated immunization with human IgG F(ab')2 fragment.

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Human IgG F(ab')2 antibody has been tested by ELISA and dot blot and is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:3,000 - 1:15,000 ELISA Dilution: 1:350,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Haltbarkeit: 12 months

Referenzen

Kameoka, Kishi, Koura, Yamakawa, Nagasawa, Ito, Matsuda, Suzuki, Nakayama, Suzuki: "Efficacy of a recombinant single-chain fragment variable region, VasSF, as a new drug for vasculitis." in: Drug design, development and therapy, Vol. 13, pp. 555-568, (2019) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Anti-Human IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the human IgG molecule. Human IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind human IgG F(c) will not bind human IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Human IgG F(ab')2 Antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.