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Kaninchen anti-Human IgG (Heavy & Light Chain) Antikörper - Preadsorbed

ELISA, IHC, WB Polyclonal IgG unconjugated
Rockland
Produktnummer ABIN101559
Hersteller Produkt- Nr.: 609-4117
  • Target Alle IgG Produkte
    IgG
    Bindungsspezifität
    • 2397
    • 515
    • 417
    • 146
    • 129
    • 128
    • 67
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 1
    • 1
    Heavy & Light Chain
    Reaktivität
    • 686
    • 680
    • 575
    • 439
    • 381
    • 208
    • 195
    • 144
    • 127
    • 126
    • 107
    • 102
    • 96
    • 88
    • 84
    • 58
    • 48
    • 34
    • 28
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 1720
    • 1430
    • 527
    • 208
    • 112
    • 111
    • 32
    • 31
    • 21
    • 18
    • 15
    • 11
    • 11
    Kaninchen
    Klonalität
    • 3146
    • 175
    • 16
    • 1
    Polyklonal
    Applikation
    • 2041
    • 1987
    • 1838
    • 1780
    • 998
    • 920
    • 896
    • 487
    • 424
    • 149
    • 98
    • 64
    • 58
    • 51
    • 45
    • 30
    • 28
    • 25
    • 20
    • 15
    • 11
    • 9
    • 8
    • 8
    • 8
    • 7
    • 7
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Hersteller Produkt- Nr.
    609-4117
    Hersteller
    Rockland
    Verwendungszweck
    Human IgG (H&L) Antibody Pre-Adsorbed
    Kreuzreaktivität (Details)
    Minimal crossreactivity against MOUSE Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG and Human Serum. No reaction was observed against Mouse Serum Proteins. Specificity was confirmed using ELISA minimal cross reaction against Mouse IgG.
    Produktmerkmale
    Anti-Human IgG (gamma chain) generated in rabbit detects human Immunoglobulin G (gamma chain).
    Aufreinigung
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    Sterilität
    Sterile filtered
    Immunogen

    Immunogen: Anti-Human IgG was produced by repeated immunization with human IgG whole molecule fragment in rabbit.

    Immunogen Type: Native Protein

    Isotyp
    IgG
  • Applikationshinweise
    Application Note: Anti-Human IgG (H&L) has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:10,000 Western Blot Dilution: 1:10,000 - 1:50,000 ELISA Dilution: 1:75,000 - 1:500,000 Other: User Optimized
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    2.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    , Preservative:0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
    Haltbarkeit
    12 months
  • Target
    IgG
    Abstract
    IgG Produkte
    Substanzklasse
    Antibody
    Hintergrund
    It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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