Kaninchen anti-Human IgG (Heavy & Light Chain) Antikörper

Produktnummer ABIN101548

Hersteller Produkt- Nr.: 209-4102

Kurzübersicht für Kaninchen anti-Human IgG (Heavy & Light Chain) Antikörper (ABIN101548)

Target: IgG

Klonalität: Polyklonal

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Human

Wirt: Kaninchen

Hersteller Produkt- Nr.: 209-4102

Hersteller: Rockland

Verwendungszweck: Human IgG (H&L) Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG and Human Serum.

Produktmerkmale: Fractalkine (CX3CL1) is an atypical chemokine that was the first of a fourth chemokine motif (CX3C). It is thought to function as a T cell and monocyte chemotractant and is produced by non-haemopoietic cells. Fractalkine is made in a soluble and membrane bound form in activated endothelial cells which is thought to promote adhesion of leukocytes. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. It is found predominantly in the small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas. Fractalkine may play a role in regulating leukocyte adhesion and migration processes at the endothelium by binding to CX3CR1. Recombinant human Fractalkine is a non-glycosylated protein with a molecular weight of 8.6 kDa. Anti-Fractalkine antibody is ideal for researchers interested in cytokines and stem cell research.

Aufreinigung: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-Human IgG whole molecule was produced by repeated immunization with Human IgG whole molecule in rabbit.

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Human IgG antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 5.0 mL

Konzentration: 10.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.