ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Isotyp
IgG
Bindungsspezifität
Fc Region
Reaktivität
Pferd
Wirt
Ziege
Hersteller Produkt- Nr.
608-1503
Hersteller
Rockland
Verwendungszweck
Horse IgG Fc Antibody Alkaline Phosphatase Conjugated
Kreuzreaktivität (Details)
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Horse IgG, Horse IgG F(c) and Horse Serum. No reaction was observed against Horse IgG F(ab).
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Application Note: Anti-Horse IgG F(c) Alkaline Phosphatase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1.0 mg/mL
Buffer
Optional[Buffer]: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Lagerung
4 °C
Informationen zur Lagerung
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Haltbarkeit
12 months
Target
IgG
Substanzklasse
Antibody
Hintergrund
Receptors bind the Fc portion of horse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.