Ziege anti-Pferd IgG (F(ab')2 Region) Antikörper (HRP)

Produktnummer ABIN101430

Hersteller Produkt- Nr.: 608-1304

Kurzübersicht für Ziege anti-Pferd IgG (F(ab')2 Region) Antikörper (HRP) (ABIN101430)

Target: IgG

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Bindungsspezifität: F(ab')2 Region

Reaktivität: Pferd

Wirt: Ziege

Hersteller Produkt- Nr.: 608-1304

Hersteller: Rockland

Verwendungszweck: Horse IgG F(ab')2 Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Horse IgG, Horse IgG F(ab')2 and Horse Serum. No reaction was observed against Horse IgG F(c).

Produktmerkmale: Anti-Horse IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Anti-Horse IgG was produced by repeated immunization with horse IgG F(ab')2 fragment in goat.

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Horse IgG F(ab')2 peroxidase conjugated antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:50,000 - 1:150,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Gamulin, Mateljak Lukačević, Lang Balija, Smajlović, Vnuk, Gulan Harcet, Tomičić, Hećimović, Halassy, Kurtović: "Pharmacokinetics of Snake Antivenom Following Intravenous and Intramuscular Administration in Envenomed Large Animal Model." in: Pharmaceutics, Vol. 17, Issue 2, (2025) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Anti-Horse F(ab')2 peroxidase conjugated antibody generated in goat detects specifically horse IgG F(ab')2. This secondary peroxidase conjugated antibody anti-Horse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.