Ziege anti-Pferd IgG (F(ab')2 Region) Antikörper

Produktnummer ABIN101422

Hersteller Produkt- Nr.: 108-1104

Kurzübersicht für Ziege anti-Pferd IgG (F(ab')2 Region) Antikörper (ABIN101422)

Target: IgG

Klonalität: Polyklonal

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Produktdetails

Bindungsspezifität: F(ab')2 Region

Reaktivität: Pferd

Wirt: Ziege

Hersteller Produkt- Nr.: 108-1104

Hersteller: Rockland

Verwendungszweck: Horse IgG F(ab')2 Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Horse IgG, Horse IgG F(ab')2 and Horse Serum.  No reaction was observed against Horse IgG F(c).

Produktmerkmale: Anti-Horse IgG Antibody generated in goat detects horse IgG.

Aufreinigung: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Optional[Immunogen]: Horse IgG F(ab')2 fragment

Anwendungsinformationen

Applikationshinweise: Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL

Konzentration: 90 mg/mL

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Anti-Horse IgG F(ab')2 Antibody generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind horse IgG F(c) will not bind horse IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.