Ziege anti-Syrischer Goldhamster IgG (Heavy & Light Chain) Antikörper (Alkaline Phosphatase (AP))
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- Target Alle IgG Produkte
- IgG
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Bindungsspezifität
- Heavy & Light Chain
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Reaktivität
- Syrischer Goldhamster
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Alkaline Phosphatase (AP)
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Applikation
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)
- Hersteller Produkt- Nr.
- 607-1502
- Hersteller
- Rockland
- Verwendungszweck
- Golden Syrian Hamster IgG (H&L) Antibody Alkaline Phosphatase Conjugated
- Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum.
- Produktmerkmale
- Anti-Golden Syrian Hamster IgG Peroxidase Antibody generated in goat detects Golden Syrian Hamster IgG.
- Aufreinigung
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian Hamster IgG coupled to agarose.
- Sterilität
- Sterile filtered
- Immunogen
- Optional[Immunogen]: Golden Syrian Hamster IgG whole molecule
- Isotyp
- IgG
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- Applikationshinweise
- Application Note: Anti-Golden Syrian Hamster IgG Alkaline Phosphatase conjugate has been tested by ELISA and dot blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:5,000 Other: User Optimized
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- Haltbarkeit
- 12 months
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- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
- Hintergrund
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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