ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Hersteller Produkt- Nr.
106-1104
Hersteller
Rockland
Verwendungszweck
Guinea Pig IgG F(ab')2 Antibody
Kreuzreaktivität (Details)
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, guinea pig IgG, guinea pig serum, Guinea Pig IgG F(ab')2. No reaction was observed against Guinea Pig IgG F(c).
Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
Konzentration
90 mg/mL
Buffer
Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Guinea Pig IgG F(ab')2 Antibody generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Guinea Pig IgG F(c) will not bind Guinea Pig IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.