ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Hersteller Produkt- Nr.
105-4103
Hersteller
Rockland
Verwendungszweck
Goat IgG Fc Antibody
Kreuzreaktivität (Details)
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Goat IgG, Goat IgG F(c) and Goat Serum. No reaction was observed against Goat IgG F(ab).
Produktmerkmale
Anti-Goat IgG Antibody generated in rabbit detects goat IgG.
Aufreinigung
This product was prepared from monospecific antiserum by a delipidation and defibrination.
Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
Konzentration
80 mg/mL
Buffer
Optional[Buffer]: None, Preservative:None
Konservierungsmittel
Without preservative
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Receptors bind the Fc portion of goat IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.