Esel anti-Ziege IgG (Heavy & Light Chain) Antikörper (HRP)

Produktnummer ABIN101187

Hersteller Produkt- Nr.: 605-703-125

Produktdetails

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Ziege

Wirt: Esel

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Hersteller Produkt- Nr.: 605-703-125

Hersteller: Rockland

Verwendungszweck: Goat IgG (H&L) Antibody Peroxidase Conjugated Pre-adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Ch GP Ham Hs Ms Rb & Rt Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Goat IgG and Goat Serum.  No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins.

Produktmerkmale: Anti-Goat IgG Peroxidase Antibody generated in donkey detects goat IgG.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Anti-Goat IgG (H&L) was produced by repeated immunization with Goat IgG whole molecule in donkey.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Goat IgG Peroxidase Conjugated Antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:170,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen

Oud, Tuijnenburg, Hempel, van Vlies, Ren, Ferdinandusse, Jansen, Santer, Johannsen, Bacchelli, Alders, Li, Davies, Dupuis, Cale, Wanders, Pals, Ocaka, James, Müller, Lehmberg, Strom, Engels, Williams et al.: "Mutations in EXTL3 Cause Neuro-immuno-skeletal Dysplasia Syndrome. ..." in: American journal of human genetics, Vol. 100, Issue 2, pp. 281-296, (2017) (PubMed).

Ellison, Gearheart, Porter, Ambruso: "IFN-γ alters the expression of diverse immunity related genes in a cell culture model designed to represent maturing neutrophils." in: PLoS ONE, Vol. 12, Issue 10, pp. e0185956, (2017) (PubMed).

Miller, Finnie, Bowden, Scholz, Oh, Kok, Burrell, Trinidad, Boyle, Li: "Preclinical efficacy studies of influenza A haemagglutinin precursor loop peptides as a potential vaccine." in: The Journal of general virology, (2011) (PubMed).

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.