Huhn anti-Ziege IgG (Heavy & Light Chain) Antikörper (HRP)

Produktnummer ABIN101163

Hersteller Produkt- Nr.: 605-9302

Kurzübersicht für Huhn anti-Ziege IgG (Heavy & Light Chain) Antikörper (HRP) (ABIN101163)

Target: IgG

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA

Isotyp: IgG

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Ziege

Wirt: Huhn

Hersteller Produkt- Nr.: 605-9302

Hersteller: Rockland

Verwendungszweck: Goat IgG (H&L) Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Chicken Serum, Goat IgG and Goat Serum.

Produktmerkmale: Anti-Goat IgG Fluorescein Antibody generated in chicken detects goat IgG.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose.

Immunogen: Optional[Immunogen]: Goat IgG whole molecule

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Goat IgG Peroxidase Antibody has been tested by ELISA and is assayed against 1.0 μg of Goat IgG in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:10,000 is suggested for this product. ELISA Dilution: 1:100 - 1:500 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.