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Kaninchen anti-Esel IgG (Heavy & Light Chain) Antikörper (HRP)

ELISA, IHC, WB Polyclonal IgG HRP
Rockland
Produktnummer ABIN101140
Hersteller Produkt- Nr.: 216-4302

Kurzübersicht für Kaninchen anti-Esel IgG (Heavy & Light Chain) Antikörper (HRP) (ABIN101140)

Target

IgG

Klonalität

  • 3453
  • 232
  • 16
  • 1
Polyklonal

Konjugat

  • 666
  • 423
  • 421
  • 400
  • 274
  • 262
  • 235
  • 119
  • 105
  • 72
  • 67
  • 64
  • 61
  • 58
  • 58
  • 50
  • 44
  • 43
  • 40
  • 40
  • 39
  • 39
  • 36
  • 36
  • 34
  • 32
  • 31
  • 30
  • 29
  • 29
  • 29
  • 29
  • 29
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 27
  • 26
  • 26
  • 25
  • 21
  • 21
  • 19
  • 17
  • 17
  • 16
HRP

Applikation

  • 2120
  • 2052
  • 1900
  • 1873
  • 999
  • 920
  • 896
  • 520
  • 424
  • 234
  • 234
  • 149
  • 102
  • 98
  • 90
  • 84
  • 51
  • 33
  • 30
  • 28
  • 25
  • 20
  • 15
  • 11
  • 9
  • 8
  • 7
  • 7
  • 5
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp

IgG
  • Bindungsspezifität

    • 2396
    • 517
    • 417
    • 146
    • 130
    • 128
    • 66
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Heavy & Light Chain

    Reaktivität

    • 753
    • 706
    • 609
    • 474
    • 410
    • 244
    • 213
    • 176
    • 146
    • 141
    • 124
    • 110
    • 107
    • 101
    • 83
    • 61
    • 48
    • 33
    • 28
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Esel

    Wirt

    • 1787
    • 1582
    • 569
    • 284
    • 122
    • 112
    • 41
    • 32
    • 22
    • 21
    • 16
    • 11
    • 11
    Kaninchen

    Hersteller Produkt- Nr.

    216-4302

    Hersteller

    Rockland

    Verwendungszweck

    Donkey IgG (H&L) Antibody Peroxidase Conjugated

    Kreuzreaktivität (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Donkey IgG, and Donkey Serum.

    Produktmerkmale

    Anti-Donkey IgG Fluorescein Antibody generated in rabbit detects donkey IgG.

    Aufreinigung

    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    Immunogen

    Optional[Immunogen]: Donkey IgG whole molecule
  • Applikationshinweise

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. This product has been assayed against 1.0 μg of Donkey IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:80,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL

    Konzentration

    10.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

    Konservierungsmittel

    Gentamicin sulfate

    Vorsichtsmaßnahmen

    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    Haltbarkeit

    12 months
  • Target

    IgG

    Substanzklasse

    Antibody

    Hintergrund

    Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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