Kaninchen anti-Huhn IgG (F(ab')2 Region) Antikörper (FITC)

Kurzübersicht für Kaninchen anti-Huhn IgG (F(ab')2 Region) Antikörper (FITC) (ABIN101063)
Target
Klonalität
Konjugat
Applikation
Isotyp
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Bindungsspezifität
- F(ab')2 Region
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Reaktivität
- Huhn
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Wirt
- Kaninchen
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Hersteller Produkt- Nr.
- 603-4204
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Hersteller
- Rockland
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Verwendungszweck
- Chicken IgG F(ab')2 Antibody Fluorescein Conjugated
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Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Chicken IgG, Chicken IgG F(ab')2 and Chicken Serum. No reaction was observed against Chicken IgG F(c).
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Produktmerkmale
- Anti-Chicken IgG F(c) Antibody detects specifically Chicken IgG F(c). It is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .
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Aufreinigung
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Immunogen
- Optional[Immunogen]: Chicken IgG F(ab')2 fragment
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Applikationshinweise
- Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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Konzentration
- 1.5 mg/mL
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Buffer
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Haltbarkeit
- 12 months
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- IgG
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Substanzklasse
- Antibody
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Hintergrund
- Anti-Chicken IgG F(ab')2 Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Chicken IgG F(c) will not bind Chicken IgG F(ab')2 Molecules.
Target
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