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Ziege anti-Rind (Kuh) IgG (F(ab')2 Region) Antikörper (FITC)

FACS, FLISA, FM Polyclonal IgG FITC
Rockland
Produktnummer ABIN100914
Hersteller Produkt- Nr.: 201-1204
  • Target Alle IgG Produkte
    IgG
    Bindungsspezifität
    • 2398
    • 515
    • 416
    • 146
    • 129
    • 128
    • 67
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 1
    • 1
    F(ab')2 Region
    Reaktivität
    • 730
    • 696
    • 598
    • 468
    • 399
    • 240
    • 208
    • 174
    • 145
    • 137
    • 112
    • 107
    • 106
    • 101
    • 84
    • 58
    • 48
    • 34
    • 28
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Rind (Kuh)
    Wirt
    • 1775
    • 1547
    • 569
    • 261
    • 112
    • 111
    • 32
    • 31
    • 21
    • 18
    • 15
    • 11
    • 11
    Ziege
    Klonalität
    • 3406
    • 182
    • 16
    • 1
    Polyklonal
    Konjugat
    • 592
    • 422
    • 421
    • 380
    • 274
    • 262
    • 235
    • 119
    • 106
    • 72
    • 67
    • 64
    • 61
    • 58
    • 58
    • 50
    • 44
    • 43
    • 40
    • 40
    • 39
    • 39
    • 36
    • 36
    • 34
    • 32
    • 31
    • 30
    • 29
    • 29
    • 29
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 27
    • 26
    • 26
    • 25
    • 21
    • 21
    • 19
    • 17
    • 17
    • 16
    FITC
    Applikation
    • 2062
    • 1988
    • 1864
    • 1807
    • 998
    • 919
    • 895
    • 487
    • 424
    • 234
    • 234
    • 149
    • 98
    • 84
    • 65
    • 58
    • 51
    • 30
    • 28
    • 25
    • 20
    • 15
    • 11
    • 9
    • 8
    • 7
    • 7
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Hersteller Produkt- Nr.
    201-1204
    Hersteller
    Rockland
    Verwendungszweck
    Bovine IgG F(ab')2 Antibody Fluorescein Conjugated
    Kreuzreaktivität (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, Bovine IgG, Bovine IgG F(ab')2 and Bovine Serum.  No reaction was observed against Bovine IgG F(c).
    Aufreinigung
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    Immunogen
    Optional[Immunogen]: Bovine IgG F(ab')2 fragment
    Isotyp
    IgG
  • Applikationshinweise
    Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:1:50,000 IF Microscopy Dilution: 1:1,000 - 1:1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
    Buffer
    Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Haltbarkeit
    12 months
  • Target
    IgG
    Abstract
    IgG Produkte
    Substanzklasse
    Antibody
    Hintergrund
    Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.
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