Ziege anti-Maus IgA, IgG, IgM Antikörper (Biotin) - Preadsorbed

Produktnummer ABIN100812

Hersteller Produkt- Nr.: 610-106-430

Kurzübersicht für Ziege anti-Maus IgA, IgG, IgM Antikörper (Biotin) - Preadsorbed (ABIN100812)

Target: IgA, IgG, IgM

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Reaktivität: Maus

Wirt: Ziege

Hersteller Produkt- Nr.: 610-106-430

Hersteller: Rockland

Verwendungszweck: Mouse IgG IgA IgM (H&L) Antibody Biotin Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Bv, Hs, Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgG, Mouse IgA and Mouse IgM.  No reaction was observed against bovine, horse or human serum proteins.  This reagent is suitable for the detection of all mouse isotypes and chain combinations.  ELISA was used to confirm specificity at less than 1 % of the target signal.

Produktmerkmale: Anti-Mouse IgG IgA IgM Biotin Antibody generated in goat detects reactivity to Mouse IgG, Mouse IgA, and Mouse IgM.

Aufreinigung: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by cross adsorption to remove unwanted specificities.

Immunogen: Optional[Immunogen]: Mouse IgG IgA and IgM whole molecule

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Mouse IgG IgA IgM Biotin Antibody has been tested by ELISA and is useful for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,500 - 1:15,000 Western Blot Dilution: 1:3,000 - 1:30,000 ELISA Dilution: 1:300,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgA, IgG, IgM

Andere Bezeichnung: IgG,IgA,IgM

Substanzklasse: Antibody

Hintergrund: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.