Ziege anti-Human IgA, IgG, IgM Antikörper (Biotin)

Produktnummer ABIN100790

Hersteller Produkt- Nr.: 609-106-130

Kurzübersicht für Ziege anti-Human IgA, IgG, IgM Antikörper (Biotin) (ABIN100790)

Target: IgA, IgG, IgM

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Reaktivität: Human

Wirt: Ziege

Hersteller Produkt- Nr.: 609-106-130

Hersteller: Rockland

Verwendungszweck: Human IgG IgA IgM (H&L) Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Goat Serum.  This product is suitable for the detection of all Human immuno-globulin classes, isotypes and chain combinations.

Produktmerkmale: Anti-Human IgG (H&L) Biotin generated in goat detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present.

Aufreinigung: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Optional[Immunogen]: Human IgG, IgA and IgM whole molecules

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Human IgG IgA IgM (H&L) Biotin Antibody has been tested by ELISA and is ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:5,000 - 1:20,000 ELISA Dilution: 1:400,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgA, IgG, IgM

Andere Bezeichnung: IgG,IgA,IgM

Substanzklasse: Antibody

Hintergrund: Anti-Human IgG IgA IgM (H&L) Biotin Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsonize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.