Kaninchen anti-Human IgA Antikörper
Kurzübersicht für Kaninchen anti-Human IgA Antikörper (ABIN100722)
Target
Klonalität
Applikation
Isotyp
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Reaktivität
- Human
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Wirt
- Kaninchen
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Hersteller Produkt- Nr.
- 609-4106
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Hersteller
- Rockland
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Verwendungszweck
- Human IgA (alpha chain) Antibody
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Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgA and Human Serum. No reaction was observed against other human heavy or light chain proteins.
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Produktmerkmale
- Anti-Human IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the human IgG molecule. Human IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind human IgG F(c) will not bind human IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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Aufreinigung
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgA coupled to agarose followed by solid phase adsorption(s).
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Sterilität
- Sterile filtered
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Immunogen
- Optional[Immunogen]: Human IgA alpha heavy chain
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Applikationshinweise
- Application Note: Anti-Human IgA Antibody is tested for ELISA and western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Immunohistochemistry Dilution: 1:250 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:5,000 - 1:20,000 Other: User Optimized
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 2.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide -
Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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Haltbarkeit
- 12 months
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- IgA
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Substanzklasse
- Antibody
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Hintergrund
- Anti-Human IgA Antibody generated in rabbit detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Target
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