Selenoprotein F Protein (SELENOF) (AA 29-165) (His tag)
Dieses Recombinant Selenoprotein F-Protein wird in Escherichia coli (E. coli) exprimiert.
Produktnummer ABIN7676652
Kurzübersicht für Selenoprotein F Protein (SELENOF) (AA 29-165) (His tag) (ABIN7676652)
Target
Selenoprotein F (SELENOF)
Protein-Typ
Recombinant
Spezies
Human
Quelle
Escherichia coli (E. coli)
Applikation
SDS-PAGE (SDS)
Reinheit
> 95 %
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Proteineigenschaft
- AA 29-165
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Aufreinigungstag / Konjugat
- Dieses Selenoprotein F Protein ist gelabelt mit His tag.
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Sequenz
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VSAFGAE FSSEACRELG FSSNLLCSSC DLLGQFNLLQ LDPDCRGCCQ EEAQFETKKL YAGAILEVCG CKLGRFPQVQ AFVRSDKPKL FRGLQIKYVR GSDPVLKLLD DNGNIAEELS ILKWNTDSVE EFLSEKLERI -
Aufreinigung
- > 95% by SDS-PAGE
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 0.5 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- Selenoprotein F (SELENOF)
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Andere Bezeichnung
- Seleno F
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Hintergrund
- SEP15 is a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the uGA codon that normally signals translation termination. The 3' uTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of uGA as a Sec codon rather than as a stop signal. Studies in mouse suggest that this selenoprotein may have redox function and may be involved in the quality control of protein folding. This gene is localized on chromosome 1p31, a genetic locus commonly mutated or deleted in human cancers. Recombinant human SEP15 (SC96C) protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molekulargewicht
- 17.7 kDa (160aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_004252
Target
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