RGL2 Antikörper
Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch RGL2 in WB. Er zeigt eine Reaktivität gegenüber Arabidopsis thaliana.
Produktnummer ABIN4966294
Kurzübersicht für RGL2 Antikörper (ABIN4966294)
Target
RGL2
(DELLA Protein RGL2 (RGL2))
Reaktivität
Arabidopsis thaliana
Wirt
Kaninchen
Klonalität
Polyklonal
Applikation
Western Blotting (WB)
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Kreuzreaktivität (Details)
- Not reactive in: Triticum sp.
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Immunogen
- KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RGL2 protein, UniProt: Q8GXW1, TAIR: At3g03450
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Applikationshinweise
- 1 : 1000 with standard ECL (WB) on transfected Arabidopsis thaliana protoplasts
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Kommentare
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As this protein is expressed in tissue in very low levels, please load at least 50 μg/well.Recommended extraction and denaturation protocol: Arabidopsis thaliana seedlings were ground in liquid nitrogen (100 μl of 2.5 x Laemli for 80-120 mg of homogenized material) and boiled for 2 min. at 95°C in 2,5x Laemmli Buffer (with 60 Mm DTT final concentration) otherwise RGA protein will degrade.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- For reconstitution add 50 μL of sterile water.
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Lagerung
- -20 °C
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Informationen zur Lagerung
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- RGL2 (DELLA Protein RGL2 (RGL2))
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Andere Bezeichnung
- RGA-like protein 2 (DELLA protein) (RGL2)
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Hintergrund
- DELLA protein RGL2 is a probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Involved in the biosynthesis of abscisic acid (ABA), delays flowering and leaf production, reulates floral development, petal, stamen and anther development. Predominantly expressed in germinating seeds, flowers and siliques. Synonymes: GRAS family protein 15, AtGRAS-15, Scarecrow-like protein 19, AtSCL19.
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Molekulargewicht
- 60 kDa
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UniProt
- Q8GXW1
Target
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