Organic Solute Transporter beta Proteine (OSTBETA)

Human Organic Solute Transporter beta (OSTBETA) Interaktionspartner

1. Dileucine motif in the extracellular N-terminal region is essential for OSTB plasma membrane targeting.

2. Hepatic OSTalpha (zeige OSTALPHA Proteine)-OSTbeta expression is induced by hypoxia.

3. OSTbeta is a target of RARalpha (zeige RARA Proteine)-mediated (by binding to DR5 (zeige TNFRSF10B Proteine) response element) gene regulation pathways

4. Ostbeta is required for both proper trafficking of Ostalpha (zeige OSTALPHA Proteine) and formation of the functional transport unit, and identify specific residues of Ostbeta critical for these processes.

5. The present report summarizes the evidence for a pleiotropic role of Ostalpha (zeige OSTALPHA Proteine)-Ostbeta in different tissues.

6. OSTbeta is localized to steroidogenic cells of the brain and adrenal gland, and it modulates DHEA/DHEAS (zeige SULT2A1 Proteine) homeostasis

7. OSTbeta has roles in biological transport and is widely expressed in human tissues

8. overexpr (zeige OSTALPHA Proteine)ession of human OSTalpha and OSTbeta facilitated the uptake of conjugated chenodeoxycholate (zeige NR1H4 Proteine) and the activation of FXR target genes

9. OSTalpha (zeige OSTALPHA Proteine)/OSTbeta expression is induced by bile acids through ligand-dependent transactivation of both OST (zeige DDOST Proteine) genes by the nuclear bile acid receptor (zeige NR1H4 Proteine)/farnesoid X receptor (zeige xpr1 Proteine) (FXR (zeige NR1H4 Proteine)).

10. the selective localization of OSTalpha (zeige OSTALPHA Proteine) and OSTbeta to the basolateral plasma membrane of epithelial cells responsible for bile acid and sterol reabsorption.

Mouse (Murine) Organic Solute Transporter beta (OSTBETA) Interaktionspartner

1. Data suggest that transport of bile acid taurocholate is retained when OstB is truncated to contain only the transmembrane domain with 15 additional residues on each side and co-expressed with intact OstA (organic solute transporter alpha subunit (zeige OSTALPHA Proteine)); shorter fragments of OstB are inactive.

2. Co-expression of mouse Ostalpha (zeige OSTALPHA Proteine)-Ostbeta, but not the individual subunits, stimulated Na(+)-independent bile acid uptake and the apical-to-basolateral transport of taurocholate

3. OSTalpha (zeige OSTALPHA Proteine) and OSTbeta mRNA levels were induced in the adrenals and kidneys of wild-type, but not FXR (zeige NR1H4 Proteine)-/-, mice

4. the selective localization of OSTalpha (zeige OSTALPHA Proteine) and OSTbeta to the basolateral plasma membrane of epithelial cells responsible for bile acid and sterol reabsorption.

5. In conclusion, we identified Ost-alpha (zeige OSTALPHA Proteine)/Ost-beta as a novel FXR (zeige NR1H4 Proteine) target. Absent Ost-alpha (zeige OSTALPHA Proteine)/Ost-beta induction in CA-fed FXR (zeige NR1H4 Proteine)(-/-) animals may contribute to increased liver injury in these animals.

6. The mouse Ostalpha (zeige OSTALPHA Proteine) and Ostbeta promoters are unusual in that they contain functional FXR (zeige NR1H4 Proteine) and LRH elements, which mediate, respectively, positive and negative feedback regulation by bile acids.

7. These results indicate that expression of Ostalpha (zeige OSTALPHA Proteine) and Ostbeta are highly regulated in response to cholestasis and that this response is dependent on the FXR bile acid receptor (zeige NR1H4 Proteine).

8. LXRalpha (zeige NR1H3 Proteine) transcriptionally regulate mouse organic solute transporter alpha (zeige OSTALPHA Proteine)/beta via inverted repeat-1 elements shared with farnesoid X receptor (zeige xpr1 Proteine)

9. Present as heterodimers (with Ost alpha (zeige OSTALPHA Proteine)) and/or heteromultimers; the interaction between Ostalpha (zeige OSTALPHA Proteine) and Ostbeta increases the stability of the proteins and is required for delivery of the heteromeric complex to the plasma membrane.

10. These data indicate that Ostalpha (zeige OSTALPHA Proteine)-Ostbeta is essential for intestinal bile acid transport in mice.