Fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay was found to be no less than 1.0 x 108 IU/mg.
Reinheit
> 96 % by SDS-PAGE and HPLC analyses.
Endotoxin-Niveau
Level Less than 1EU/µg of rHuIFNalpha1b as determined by LAL method
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
HPLC, SDS
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end. Synonym: Interferon-alpha1b (IFN-alpha1b), Human. Formulation: Lyophilized from a 0.2µm filtered solution in PBS, pH 7.4, containing 4% mannitol and 1% HSA.
Molekulargewicht
Approximately 19.5 kDa, a single non-glycosylated polypeptide chain containing 167 amino acids.