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IFN alpha 2b Protein

This anti-IFN alpha 2b antibody (ABIN987980) is a Escherichia coli (E. coli) antibody detecting IFN alpha 2b in . Suitable for Human.
Produktnummer ABIN987980

Kurzübersicht für IFN alpha 2b Protein (ABIN987980)

Target

IFN alpha 2b

Protein-Typ

Recombinant

Biologische Aktivität

Active

Spezies

Human

Quelle

  • 2
  • 1
  • 1
Escherichia coli (E. coli)

Reinheit

> 95.0 % as determined by:(a) Analysis by RP-HPLC.(b) Anion-exchange FPLC.(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
  • Sequenz

    analysis The sequence of the first five N-terminal amino acids was determined and was found to be Met-Cys-Asp-Leu-Pro. N-terminal methionine has been completely removed enzymatica lly. p to grams level.

    Produktmerkmale

    The specific activity as determined in a viral resistance assay using VSV-WISH cells was found to be 1.0×108IU/mg.

    Endotoxin-Niveau

    Level Less than 0.1 ng/µg (IEU/µg) of Recombinant Human Interferon alpha 2b (IFN alpha2b
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  • Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    It is recommended to reconstitute the lyophilized Recombinant Human Interferon alpha 2b (IFN alpha2b) in sterile 18M-omega-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. Quantitation Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a calibrated solution of IFN-alpha 2b as a Reference Standard.

    Lagerung

    -20 °C
  • Perales, Beach, Gallego, Soria, Quer, Esteban, Rice, Domingo, Sheldon: "Response of hepatitis C virus to long-term passage in the presence of alpha interferon: multiple mutations and a common phenotype." in: Journal of virology, Vol. 87, Issue 13, pp. 7593-607, (2013) (PubMed).

  • Target

    IFN alpha 2b

    Andere Bezeichnung

    IFN-alpha 2b

    Hintergrund

    Human Interferon alpha 2b (IFN alpha2b) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19,269 Da. The Interferon alpha 2b (IFN alpha2b) gene was obtained from human leukocytes. The Recombinant Human Interferon alpha 2b (IFN alpha2b) is purified by proprietary chromatographic techniques. Synonym: rHuIFN-alpha-2b, rHuIFNalpha-2b, rHuIFN-a-2b, rHuIFN-alfa-2b, rHuIFNalfa-2b, Interferon-alpha-2b, Interferonalpha-2b, Interferon-a-2b, Interferon-alfa-2b, Interferonalfa-2. Formulation: Lyophilized from a (1 mg/ml) solution in containing 2.3 mg Sodium phosphate dibasic and 0.55 mg sodium phosphate monobasic buffer.

    Molekulargewicht

    19.4kDa +/- 10% Isoelectric Point The main zone between 5.5-6.8 analysis by IEF. UV Scan The maximal absorption wave is 278+/-3 nm.
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