Protein A Protein (HRP)

Produktnummer ABIN967967

Dieses Native Protein A-Protein wird in Staphylococcus aureus produziert.

Kurzübersicht für Protein A Protein (HRP) (ABIN967967)

Target: Protein A

Protein-Typ: Native

Spezies: Staphylococcus aureus

Quelle: Staphylococcus aureus

Applikation: Western Blotting (WB)

Produktdetails

Aufreinigungstag / Konjugat: Dieses Protein A Protein ist gelabelt mit HRP.

Produktmerkmale: Protein A has been reported to bind strongly to rabbit IgG and mouse IgG2 isotypes. However, protein A does not bind mouse IgM and weakly binds IgG1. When conjugated to agarose, protein A acts as a solid matrix facilitating the immunoprecipitation of antigen-antibody complexes. It can also be utilized for antibody purification. As a horseradish peroxidase conjugate, protein A can be used instead of secondary antibody detection methods in Western blotting with polyclonal antibodies and with IgG2a and IgG2b mouse monoclonal antibodies.
Horseradish peroxidase (HRP) was coupled to protein A at a concentration of 4 mg/mg of protein A using the periodate method. For the detection of antigen using polyclonal antibodies in Western blotting, a concentration of 0.5 µg/ml (1:2000 dilution) is suggested.

Aufreinigung: Purified from tissue culture supernatant or ascites by affinity chromatography.

Anwendungsinformationen

Kommentare:

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 50 % glycerol, 20 mM sodium phosphate pH 7.5, 150 mM NaCl.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Antigendetails

Target: Protein A

Andere Bezeichnung: ProteinA