Streptavidin Protein (HRP)

Produktnummer ABIN964537

Hersteller Produkt- Nr.: s000-03

Native Streptavidin-Protein exprimiert in Streptomyces avidinii und wurde in 4+ Publikationen erwähnt.

Kurzübersicht für Streptavidin Protein (HRP) (ABIN964537)

Target: Streptavidin

Protein-Typ: Native

Spezies: Streptomyces avidinii

Quelle: Streptomyces avidinii

Applikation: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Dot Blot (DB), Immunofluorescence (IF), Immunoelectron Microscopy (IEM)

Produktdetails

Aufreinigungstag / Konjugat: Dieses Streptavidin Protein ist gelabelt mit HRP.

Hersteller Produkt- Nr.: s000-03

Hersteller: Rockland

Verwendungszweck: Streptavidin Peroxidase Conjugated

Aufreinigung: Streptavidin-HRP was prepared from chromatographically purified streptavidin.  Streptavidin Peroxidase conjugate was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin.

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Streptavidin Peroxidase has been tested by dot blot and western blot and is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in Immunohistochemistry, Immunofluorescence, immuno-EM, Western Bl

Western Blot Dilution: 1:10,000 - 1:40,000

ELISA Dilution: 1:20,000 - 1:200,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Restore with deionized water (or equivalent) (in 1.0 mL)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do NOT add Sodium Azide! Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Streptavidin Peroxidase Expiration date is one (1) year from date of receipt.

Haltbarkeit: 12 months

Referenzen

Metzner, Luckert, Lemuth, Hämmerle, Moos: "Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection." in: Sensors (Basel, Switzerland), Vol. 18, Issue 5, (2018) (PubMed).

Vasek, Garber, Dorsey, Durrant, Bollman, Soung, Yu, Perez-Torres, Frouin, Wilton, Funk, DeMasters, Jiang, Bowen, Mennerick, Robinson, Garbow, Tyler, Suthar, Schmidt, Stevens, Klein: "A complement-microglial axis drives synapse loss during virus-induced memory impairment." in: Nature, Vol. 534, Issue 7608, pp. 538-43, (2016) (PubMed).

Cantoni, Bollman, Licastro, Xie, Mikesell, Schmidt, Yuede, Galimberti, Olivecrona, Klein, Cross, Otero, Piccio: "TREM2 regulates microglial cell activation in response to demyelination in vivo." in: Acta neuropathologica, Vol. 129, Issue 3, pp. 429-47, (2015) (PubMed).

Cowles, Li, Semmelhack, Cristea, Silhavy: "The free and bound forms of Lpp occupy distinct subcellular locations in Escherichia coli." in: Molecular microbiology, Vol. 79, Issue 5, pp. 1168-81, (2011) (PubMed).

Antigendetails

Target: Streptavidin

Hintergrund:

Synonyms: HRP-SA, Horseradish Peroxidase conjugated S avidin, Streptavidin HRP, Streptavidin conjugated to horseradish peroxidase (HRP), HRP-linked Streptavidin

Background: Streptavidin is isolated from bacteria, Streptomyces avidinii, and has an exceptionally high binding affinity for B7 (biotin). Rockland offers streptavidin in unconjugated and conjugated forms for common immunoassays including ELISA, western blotting, im

UniProt: P22629