Protein A Protein (Sepharose)

Produktnummer ABIN964524

Hersteller Produkt- Nr.: pa50-00-0002

Dieses Native Protein A-Protein wird in exprimiert.

Kurzübersicht für Protein A Protein (Sepharose) (ABIN964524)

Target: Protein A

Protein-Typ: Native

Spezies: Staphylococcus aureus

Wirt: Bitte anfragen

Applikation: Immunoprecipitation (IP)

Produktdetails

Aufreinigungstag / Konjugat: Dieses Protein A Protein ist gelabelt mit Sepharose.

Hersteller Produkt- Nr.: pa50-00-0002

Hersteller: Rockland

Produktmerkmale: Concentration Definition: drained sepharose gel (~1 cc settled sepharose beads per 2 ml suspension)
Performance Data: Bead size 45-165 µm in size when swollen. ~35 mg Human IgG bound per cc of drained beads.

Anwendungsinformationen

Applikationshinweise: Protein A resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction.

Kommentare:

Sepharose 4 Fast Flow from GE/Amersham-Pharmacia

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 6.0 mg/cc

Buffer: 20% (v/v) Ethanol

Lagerung: 4 °C

Antigendetails

Target: Protein A

Hintergrund: Sepharose™ Protein A is a suspension of beads conjugated to a fusion protein containing binding domains from protein A from Staphylococcus aureaus. When evenly dispersed approximately 2.0 cc of suspension is equal to 1.0 cc of settled beads of Sepharose™ 4 Fast Flow (highly cross linked, 4% agarose derivative). The bead size is approximately 45-165 µm when swollen. Approximately 6.0 mg of Protein A is bound per cc of drained beads. Each cc of drained beads will bind approximately 35 mg Human. The exclusion limit (Mr) of the beads is 3 x 10^7. The maximum linear flow rate is approximately 1300 cm/hr. This product shows long term stability within pH range of 3 to 9 and short term stability within pH range of 2 to 10.
Synonyms: Sepharose Protein A