Biotin Protein (Glucose Oxidase)

Produktnummer ABIN964126

Hersteller Produkt- Nr.: b000-07

Biotin-Protein exprimiert in Synthetic.

Kurzübersicht für Biotin Protein (Glucose Oxidase) (ABIN964126)

Target: Biotin

Spezies: Chemical

Quelle: Synthetic

Applikation: Dot Blot (DB), ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Microarray (MA)

Produktdetails

Aufreinigungstag / Konjugat: Dieses Biotin Protein ist gelabelt mit Glucose Oxidase.

Hersteller Produkt- Nr.: b000-07

Hersteller: Rockland

Verwendungszweck: Biotin Glucose Oxidase Conjugated

Aufreinigung: Biotin Glucose Oxidase Conjugated was prepared from chromatographically purified biotin.  Biotin Glucose Oxidase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Glucose Oxidase and anti-Biotin.

Sterilität: Sterile filtered

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Biotin Glucose Oxidase Conjugated has been tested by ELISA and dot blot and can be utilized in ELISA and Western Blotting experiments where the assay's target of interest is coupled with streptavidin.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:8,000 - 1:32,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Restore with deionized water (or equivalent) (in 5.0 mL)

Konzentration: 1.0 mg/mL

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handhabung: Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Biotin Glucose Oxidase Expiration date is one (1) year from date of receipt.

Haltbarkeit: 12 months

Antigendetails

Target: Biotin

Substanzklasse: Chemical

Hintergrund:

Synonyms: Biotin Glucose Oxidase Conjugation, Glucose Oxidase Conjugated Biotin, Biotin conjugated Glucose Oxidase

Background: Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, Biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptav