Biotin Protein (HRP)

Produktnummer ABIN964124

Hersteller Produkt- Nr.: b000-03

Dieses Biotin-Protein wird in Synthetic produziert.

Kurzübersicht für Biotin Protein (HRP) (ABIN964124)

Target: Biotin

Spezies: Chemical

Quelle: Synthetic

Applikation: Dot Blot (DB), ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Produktdetails

Aufreinigungstag / Konjugat: Dieses Biotin Protein ist gelabelt mit HRP.

Hersteller Produkt- Nr.: b000-03

Hersteller: Rockland

Verwendungszweck: Biotin Peroxidase Conjugated

Aufreinigung: Biotin Peroxidase Conjugated was prepared from chromatographically purified biotin. Biotin Peroxidase Conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Biotin.

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Biotin Peroxidase Conjugated has been tested by ELISA and dot blot and can be utilized in ELISA and Western Blotting experiments where the assay's target of interest is coupled with streptavidin.

Western Blot Dilution: 1:10,000 - 1:40,000

ELISA Dilution: 1:20,000 - 1:200,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Restore with deionized water (or equivalent) (in 5.0 mL)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do NOT add Sodium Azide! Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Biotin Peroxidase Conju Expiration date is one (1) year from date of receipt.

Haltbarkeit: 12 months

Antigendetails

Target: Biotin

Substanzklasse: Chemical

Hintergrund:

Synonyms: Vitamin H, Coenzyme R, Vitamin B7, Biotin Peroxidase Conjugated, Biotin HRP Conjugated

Background: Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptav