Purified recombinant Human SCGF beta protein Expression System: E.coli Bioactivity: Determined by its inhibitory effect on the proliferation of TF-1 cells previously grown in media containing GM-CSF. The ED50 for this effect was observed at 7 ng/mL.
Reinheit
> 98 % pure
Sterilität
Sterile filtered
Endotoxin-Niveau
< 0.1 ng per µg (1 EU/µg).
Applikationshinweise
Each Investigator should determine their own optimal working dilution for specific applications.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute with water to a concentration of 5 g/mL.
Buffer
Lyophilized from sterile filtered 0.25X PBS with 1 mM DTT and no additives.
Konservierungsmittel
Dithiothreitol (DTT)
Vorsichtsmaßnahmen
This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handhabung
Avoid repeated freeze/thaw cycles.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
Target
SCGF beta
Hintergrund
SCGF a and beta are hematopoietic growth factor that exerts their activity at early stages of hematopoiesis. The SCGFs are non-glycosylated species-specific cytokines that can support growth of primitive hematopoietic cells, and in combination with EPO or GM-CSF, promote proliferation or erthroid or myeloid progenitors, respectively. Alternative Names: Stem Cell Growth Factor-beta protein