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Thioredoxin Reductase Protein (TrxR) (AA 1-321)

Recombinant Thioredoxin Reductase-Protein exprimiert in Escherichia coli (E. coli).
Produktnummer ABIN935101

Kurzübersicht für Thioredoxin Reductase Protein (TrxR) (AA 1-321) (ABIN935101)

Target

Alle Thioredoxin Reductase (TrxR) Proteine anzeigen
Thioredoxin Reductase (TrxR)

Protein-Typ

Recombinant

Biologische Aktivität

Active

Spezies

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E. coli

Quelle

  • 12
  • 1
Escherichia coli (E. coli)

Applikation

SDS-PAGE (SDS)

Reinheit

> 90 % pure
  • Proteineigenschaft

    AA 1-321

    Sequenz

    MGTTKHSKLL ILGSGPAGYT AAVYAARANL QPVLITGMEK GGQLTTTTEV ENWPGDPNDL TGPLLMERMH EHATKFETEI IFDHINKVDL QNRPFRLNGD NGEYTCDALI IATGASARYL GLPSEEAFKG RGVSACATCD GFFYRNQKVA VIGGGNTAVE EALYLSNIAS EVHLIHRRDG FRAEKILIKR LMDKVENGNI ILHTNRTLEE VTGDQMGVTG VRLRDTQNSD NIESLDVAGL FVAIGHSPNT AIFEGQLELE NGYIKVQSGI HGNATQTSIP GVFAAGDVMD HIYRQAITSA GTGCMAALDA ERYLDGLADA K

    Produktmerkmale

    Purified recombinant E.coli TRXB protein
    Expression System: E.coli
    Bioactivity: Specific activity is 4-5 units/mL, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm.
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  • Applikationshinweise

    TRXB protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

    Testdurchführung

    1. Prepare a 0.7 mL reaction mixture into a suitable container: The final concentrations are 107 mM potassium phosphate, 17 mM EDTA, 0.7 mM beta-NADPH, 0.05 % BSA, 0.014 % (w/v) thioredoxin, 7 mM DTNB, 5 µg, E. coli TRXB.
      2. Equilibrate to 25 °C and monitor the A412 nm until the value is constant using a spectrophotometer.
      3. Add 35 µL of 107 mM DTNB into reaction mixture and mix immediately.
      4. Record the increase in A412 nm for 2 minutes.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 mg/mL

    Buffer

    Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT and 10 % glycerol.

    Konservierungsmittel

    Dithiothreitol (DTT)

    Vorsichtsmaßnahmen

    This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Handhabung

    Avoid repeated freeze/thaw cycles.

    Lagerung

    RT/-20 °C

    Informationen zur Lagerung

    Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
  • Target

    Thioredoxin Reductase (TrxR)

    Andere Bezeichnung

    TRXB

    Hintergrund

    TRXB(Thioredoxin reductase) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidative stress. The mammalian Thioredoxin reductase reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. Recombinant E. coli TRXB protein was expressed in E. coli and purified by using conventional chromatography techniques.
    Alternative Names: protein, Thioredoxin reductase protein, FAD/NAD(P)-binding protein, Thioredoxin reductase JW0871 protein, TRXR., ECK0879 protein

    Molekulargewicht

    34.6 kDa (321 AA)
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