Glucose-6-Phosphate Dehydrogenase Protein (G6PD) (AA 1-491)

Produktnummer ABIN935018

Dieses Recombinant Glucose-6-Phosphate Dehydrogenase-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für Glucose-6-Phosphate Dehydrogenase Protein (G6PD) (AA 1-491) (ABIN935018)

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: E. coli

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 90 % pure

Produktdetails

Proteineigenschaft: AA 1-491

Sequenz: MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E

Produktmerkmale: Purified recombinant E.coli G6 PD protein
Expression System: E.coli
Bioactivity: Specific activity is 8-10 units/mL obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 m D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25 °C.
Molecular weight on SDS-PAGE will appear higher.

Anwendungsinformationen

Applikationshinweise: G6PD protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Testdurchführung:

1. Prepare a 3 mL reaction cocktail into a suitable container: The final concentrations are 53 mM gylcylglycine, 3 mM D-glucose 6-phosphate, 0.63 mM beta-NADP, 13 mM MgCl2.
   2. Equilibrate to 25 °C and monitor at A340 nm until the value is constant using a spectrophotometer.
   3. Add 4 µg of recombinant G6PD into reaction cocktail and mix immediately.
   4. Record the increase at A340 nm for 5 minutes.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 50 mM MES 6.0, 0.0 mM PMSF, 0 mM EDTA, 0.0 mM DTT, and 10 % glycerol.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: RT/-20 °C

Informationen zur Lagerung: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Antigendetails

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Andere Bezeichnung: G6PD

Hintergrund: Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphogluconolactone and simultaneously produces NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia. Recombinant G6PD protein was expressed in E. coli and purified by conventional chromatography techniques.
Alternative Names: Glucose 6 phosphate 1 dehydrogenase protein, G6PD1 protein, zwf protein, GPD 6, Glucose-6-phosphate 1-dehydrogenase G6PD protein, Glucose-6-phosphate 1-dehydrogenase protein, G6PD, POS10 protein, GPD-6, GPD-6 protein, MET19 protein, GPD 6 protein, G6pdx protein, Zwf1p.

Molekulargewicht: 55.7 kDa (491 AA)

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones